In our first blog post, we described the fantastic signal quality of the Open Ephys acquisition system. Today, we made our first direct comparison between signals recorded with Open Ephys and those recorded with a Neuralynx Digital Lynx system. The conclusion? The recordings are virtually indistinguishable. There's still more rigorous testing to be done, but to the trained eye of a seasoned electrophysiologist, the signal quality of the two systems is qualitatively identical. Read on to see for yourself.
We performed the test by recording from a mouse implanted with an eight tetrode flexDrive. The Omnetics connector on the electrode interface board was compatible with both the Open Ephys headstage and a special-ordered Neuralynx HS-36.
The mouse was placed in a 1' x 1' arena, and attached to either recording system via a tether that was counter-balanced via a system of pulleys.
We made sure the settings on each system were the same (1 to 7500 Hz bandpass, ~30 kHz sampling rate), then recorded for 3 minutes with Open Ephys and 3 minutes with Neuralynx
The examples below all come from a single electrode, but the signals across all 32 channels are identical between the two systems, as far as we can tell. We hope to carry out a more detailed comparison in the near future, but we couldn't be happier with the initial results!