Wire-Free Miniscope Modification Kit (CMOS PCB, Focus Slider, Battery, SD Card)
Wire-Free Miniscope Modification Kit (CMOS PCB, Focus Slider, Battery, SD Card)
Note: This product has been declared obsolete and will be available for purchase until all remaining units have been sold.
This kit has all you need to convert the wired Miniscope system v3 into wire-free using the focus slider and CMOS PCB.
It still requires the main body, filter cover, filters, and optics from the original wired v3 Miniscope.
Further information can be found here.
The Wire-Free Miniscope Modification Kit includes:
1x Wire-Free Miniscope CMOS Imaging Sensor PCB fully assembled and tested
1x Wire-Free Miniscope Focus Slider
1x 45 mAh battery with connector (charger not included)
1x Micro SD card
1x Miniscope Excitation LED PCB (assembled)
For your convenience, the excitation LED is shipped already soldered to the CMOS PCB. The excitation LED can also be provided separately. If you need just the excitation LED with flexible silicon cable already attached, please send an inquiry to info@oeps.tech
If you need a formal quote and/or prefer bank transfer as the payment method, please contact us via info@oeps.tech.
Attention: If you are planing to order Wire-Free Batteries, shipping restrictions are applied.
Please do not forget to check the Wire-Free Battery page for extended information.
Stay up to date: Updated information from the Miniscope team can be found at Miniscope website. You would ned to trough a quick registration process in order to have access to the content.
Design files and source code:
https://github.com/daharoni/wire-free-miniscope
General information.
- The wire-free Miniscope optics are very similar to the v3 Miniscope. You can use the same Miniscope body, cover, baseplates, and magnets. The two systems are completely interchangeable on the animal.
- The wire-free Miniscope's gain, excitation LED power, and recording length is configured on power-up once an microSD card is detected by the on board’s microcontroller. The microcontroller checks specific memory locations in the microSD card to load the configuration. Configurations are initially uploaded onto the microSD card using the MiniscopeSDCardReader [LINK TO] software.
- Once the wire-free Miniscope has been powered up and a microSD card has been detected, the Miniscope waits 5 seconds then begins recording at 20FPS. At the start of recording the red status LED on the wire-free PCB will light up. Once recording has finished the red status LED will turn off. You can use the on and off timing of this LED to synchronize wire-free Minsicope recordings with an external behavioral camera.
- The last 12 pixels values of each frame recorded to the microSD card are over written with a footer that contains four 32bit values. The second to last 32bit value contains the time stamp of that frame. This timestamp is in milliseconds and relative to the start of recording. This timestamp was validated and is extremely stable across 25 minute recordings. Below is an example code snippet of how you can reconstruct the timestamp for a give frame 'frame' in MATLAB:
footer = (frame(end,(end-7):end)); timestamp = footer(1) + bitshift(footer(2),8) + bitshift(footer(3),16) + bitshift(footer(4),24);
- The wire-free system will run on any single cell lipo battery (and more generally, with anything supplying between 4.2V and 3.4V that is capable of supplying ~80mA). With a 45mAh single cell lipo battery you can expect to get slightly over 20 minutes of recording. The suggested workflow is to first image the animals with a wired Miniscope to get an estimate of focal depth, gain, and excitation LED power. Set these parameters on the wire-free Miniscope and then do short recordings, checking the resulting video each time, while adjusting the parameters until you have achieved good imaging. In our experience, once you find the optimal parameters you should be able to keep those same parameters across weeks.